Diagnosis of Mycoplasma bovis Infection in Cattle by ELISA and PCR
نویسندگان
چکیده
منابع مشابه
Detection of Babesia bovis in cattle by PCR-ELISA.
We established a new highly sensitive method, PCR-ELISA, for the dectection of Babesia bovis in cattle for farms in Thailand. The detection of around 2.4 x 10(-8)% parasitemia (equivalent to 1 infected erythrocyte per 2 ml) was achieved by PCR amplification followed by the ELISA detection of a biotin tagged gene. When comparing the sensitivity of PCR-ELISA with the microscopic method, our PCR-E...
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Neospora caninum, an apicomplexan protozoan, is regarded as a major cause of abortion and stillbirth in cattle in countries world-wide. The ability to detection N.caninum in tissue samples can be a useful detection diagnostic tool for use in the study of the pathogenicity, immunoprophylaxis, and treatment of Neospora infection. However, molecular biology is one of the most sensitive tools for d...
متن کاملDesigning and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this...
متن کاملMycoplasma bovis infections in cattle.
Mycoplasma bovis is a pathogen causing respiratory disease, otitis media, arthritis, mastitis, and a variety of other diseases in cattle worldwide. It is increasingly recognized by the veterinary and livestock communities as having an important impact on the health, welfare, and productivity of dairy and beef cattle. M. bovis diseases can be difficult to diagnose and control because of inconsis...
متن کاملDevelopment of a recombinant antigen for antibody-based diagnosis of Mycoplasma bovis infection in cattle.
Mycoplasma bovis induces various clinical manifestations in cattle, such as mastitis, arthritis, and pneumonia. We have evaluated the immunoreactivity of three variable surface proteins (Vsps) of M. bovis, namely VspA, VspB, and VspC, with sera collected from herds with mycoplasmosis or from cattle experimentally infected with M. bovis. Western blot analysis revealed that the Vsps are the predo...
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ژورنال
عنوان ژورنال: Kafkas Universitesi Veteriner Fakultesi Dergisi
سال: 2014
ISSN: 1300-6045
DOI: 10.9775/kvfd.2013.9959